Stem Cell Mobilization: Methods and Protocols - Methods in Molecular Biology 2012 edition

Marc Notes: Includes bibliographical references and index. Brief Description: This volume in the Methods in Molecular Biology series details cell mobilization methodology anddescribes recent developments in the field of basic and biomedical research. Offers materials and reagents lists, step-by-step protocols and troubleshooting tips."Table of Contents:1. Mobilization of Hematopoietic Stem/progenitor Cells: General Principles and Molecular MechanismsHalvard Bonig and Thalia Papayannopoulou2. Quantifying Hematopoietic Stem and Progenitor Cell MobilizationShiri Gur-Cohen, Kfir Lapid, Tsvee Lapidot3. Hematopoietic Stem Cell Mobilization with G-CSF Chitra Hosing4. Hematopoietic Stem Cell Mobilization with Agents Other Than G-CSFJonathan Hoggatt and Louis M. Pelus5. Hematopoietic Stem Cell Mobilization: A Clinical ProtocolGina Pesek and Michele Cottler-Fox6. Monitoring Blood for CD34+ cells to Determine Timing of Hematopietic Progenitor Cells (HPC) ApheresisM. Louette Vaughn and Edmund K. Waller7. Hematopoietic Progenitor Cell CollectionS. Darlene Marlow and Myra House8. Managing Apheresis Complications during the Hematopoietic Stem Cell CollectionS. Darlen Marlow and Myra House9. Hematopoietic Progenitor Cell Apheresis Processing Eleanor S. Hamilton and Edmund K. Waller10. Toxicities of Mobilized Stem Cell Infusion Jonathan L. Kaufman11. Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow MacrophagesValerie Barbier Ingrid G. Winkler and Jean-Pierre Levesque 12. Combinatorial Stem Cell Mobilization in Animal ModelsSimon C Pitchford and Sara M Rankin13. Vascular Progenitor Cell Mobilization Kirsten A. Kienstra and Karen K. Hirschi14. Evaluation of Circulating Endothelial Precursor Cells in Cancer PatientsFrancesco Bertolini, Patrizia Mancuso, Liat Benayoun, Svetlana Gingis-Velitski, and Yuval Shaked15. Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells Erika L. Spaeth, Shannon Kidd and Frank C Marini16. Differentiation of Circulating Monocytes into Fibroblast-like CellsDarrell Pilling and Richard H. Gomer17. Enumeration of Very Small Embryonic Like Stem Cells in Peripheral BloodRui Liu and Mariusz Z. Ratajczak 18. Generation of Vascular Niche for Studying Stem Cell Homeostasis Jason M. Butler and Shahin Rafii19. Studying Vascular Progenitor Cells in a Neonatal Mouse Model Kirsten A. Kienstra and Karen K. Hirschi20. Progenitor Cell Mobilization from Extramaedullary OrgansMikhail G. Kolonin"Jacket Description/Back: Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells. In" Stem Cell Mobilization: Methodsand Protocols," expert researchers in the field detail cell mobilization methodology and recent developments in the field for basic and biomedical research community. Specifically clinical hematopoietic progenitor cell mobilization protocols, frontiers in mobilization and analysis of non-hematopoietic progenitors, mesenchymal progenitor cells, monocyte-derived fibroblast progenitors, and very small embryonic like cells. Written in the highly successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "Stem Cell Mobilization: Methods and Protocols "seeks to aid scientists in the further study of this important stem cell research. "